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Colony Lift Colorimetric Assay for β-Galactosidase Activity
In this protocol, we present a qualitative assay for monitoring the level of expression of β-galactosidase, an enzyme encoded by the LacZ gene, in yeast. This is useful both for determining autoactivity of LacZ expression in yeast DNA “bait” strains and for assessing LacZ reporter gene activation me...
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| Опубликовано в: : | Cold Spring Harb Protoc |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2016
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5458599/ https://ncbi.nlm.nih.gov/pubmed/27934685 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot088963 |
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