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Zymolyase-Treatment and Polymerase Chain Reaction Amplification from Genomic and Plasmid Templates from Yeast

Here, we present a protocol for amplifying DNA fragments from the genome of, or plasmids transformed into, yeast strains that require the use of the lytic enzyme zymolyase to break open the yeast cells by digesting the cell wall. Yeast strains requiring such treatment include YM4271 and Y1HaS2, wher...

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Detaylı Bibliyografya
Yayımlandı:Cold Spring Harb Protoc
Asıl Yazarlar: Fuxman Bass, Juan I., Reece-Hoyes, John S., Walhout, Albertha J.M.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2016
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5458600/
https://ncbi.nlm.nih.gov/pubmed/27934686
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot088971
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