Detection of individual fluorescently labeled reovirions in living cells.
Reovirus serotype 1 (Lang) can be conjugated with rhodamine B or fluorescein isothiocyanate in a way that preserves viral infectivity. We have used epifluorescence microscopy to detect individual virions bound to the surface of cells and to follow in real time the early stages of reovirus infection...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54580/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2118653/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.17.6579 |
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