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Detection of individual fluorescently labeled reovirions in living cells.

Reovirus serotype 1 (Lang) can be conjugated with rhodamine B or fluorescein isothiocyanate in a way that preserves viral infectivity. We have used epifluorescence microscopy to detect individual virions bound to the surface of cells and to follow in real time the early stages of reovirus infection...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Autores principales: Georgi, A, Mottola-Hartshorn, C, Warner, A, Fields, B, Chen, L B
Formato: Artigo
Lenguaje:Inglês
Publicado: National Academy of Sciences 1990
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54580/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2118653/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.17.6579
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