A genome annotation-driven approach to cloning the human ORFeome

We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones repres...

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書誌詳細
主要な著者: Collins, John E, Wright, Charmain L, Edwards, Carol A, Davis, Matthew P, Grinham, James A, Cole, Charlotte G, Goward, Melanie E, Aguado, Begoña, Mallya, Meera, Mokrab, Younes, Huckle, Elizabeth J, Beare, David M, Dunham, Ian
フォーマット: Artigo
言語:Inglês
出版事項: BioMed Central 2004
主題:
Method
オンライン･アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC545604/
https://ncbi.nlm.nih.gov/pubmed/15461802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2004-5-10-r84
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