A genome annotation-driven approach to cloning the human ORFeome

We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones repres...

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Main Authors: Collins, John E, Wright, Charmain L, Edwards, Carol A, Davis, Matthew P, Grinham, James A, Cole, Charlotte G, Goward, Melanie E, Aguado, Begoña, Mallya, Meera, Mokrab, Younes, Huckle, Elizabeth J, Beare, David M, Dunham, Ian
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2004
Assuntos:
Method
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC545604/
https://ncbi.nlm.nih.gov/pubmed/15461802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2004-5-10-r84
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