A genome annotation-driven approach to cloning the human ORFeome

We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones repres...

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Autors principals: Collins, John E, Wright, Charmain L, Edwards, Carol A, Davis, Matthew P, Grinham, James A, Cole, Charlotte G, Goward, Melanie E, Aguado, Begoña, Mallya, Meera, Mokrab, Younes, Huckle, Elizabeth J, Beare, David M, Dunham, Ian
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2004
Matèries:
Method
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC545604/
https://ncbi.nlm.nih.gov/pubmed/15461802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2004-5-10-r84
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