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A reverse genetic screen in Drosophila using a deletion-inducing mutagen
We report the use of the cross-linking drug hexamethylphosphoramide (HMPA), which introduces small deletions, as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster. A compatible mutation-detection method based on resolution of PCR fragment-length polymorphisms on s...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC545603/ https://ncbi.nlm.nih.gov/pubmed/15461801 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2004-5-10-r83 |
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