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Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time

Here we present a technique called single-molecule high-resolution colocalization (SHREC) of fluorescent dyes that allows the measurement of interfluorophore distances in macromolecules and macromolecular complexes with better than 10-nm resolution. By using two chromatically differing fluorescent m...

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Main Authors: Churchman, L. Stirling, Ökten, Zeynep, Rock, Ronald S., Dawson, John F., Spudich, James A.
Formáid: Artigo
Teanga:Inglês
Foilsithe: National Academy of Sciences 2005
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC545495/
https://ncbi.nlm.nih.gov/pubmed/15668396
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0409487102
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