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Determination of the distance between two spin labels attached to a macromolecule.

An EPR "spectroscopic ruler" was developed using a series of alpha-helical polypeptides, each modified with two nitroxide spin labels. The EPR line broadening due to electron-electron dipolar interactions in the frozen state was determined using the Fourier deconvolution method. These dipo...

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Bibliographic Details
Main Authors: Rabenstein, M D, Shin, Y K
Format: Artigo
Language:Inglês
Published: 1995
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC41132/
https://ncbi.nlm.nih.gov/pubmed/7667275
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