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Optimized localization-analysis for single-molecule tracking and super-resolution microscopy

We optimally localize isolated fluorescent beads and molecules imaged as diffraction-limited spots, determine the orientation of molecules, and present reliable formulae for the precisions of various localization methods. For beads, theory and experimental data both show that unweighted least-square...

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Hlavní autoři: Mortensen, Kim I., Churchman, L. Stirling, Spudich, James A., Flyvbjerg, Henrik
Médium: Artigo
Jazyk:Inglês
Vydáno: 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3127582/
https://ncbi.nlm.nih.gov/pubmed/20364147
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1447
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