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Optimized localization-analysis for single-molecule tracking and super-resolution microscopy
We optimally localize isolated fluorescent beads and molecules imaged as diffraction-limited spots, determine the orientation of molecules, and present reliable formulae for the precisions of various localization methods. For beads, theory and experimental data both show that unweighted least-square...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3127582/ https://ncbi.nlm.nih.gov/pubmed/20364147 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1447 |
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