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Optimized localization-analysis for single-molecule tracking and super-resolution microscopy
We optimally localize isolated fluorescent beads and molecules imaged as diffraction-limited spots, determine the orientation of molecules, and present reliable formulae for the precisions of various localization methods. For beads, theory and experimental data both show that unweighted least-square...
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Main Authors: | , , , |
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格式: | Artigo |
語言: | Inglês |
出版: |
2010
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主題: | |
在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3127582/ https://ncbi.nlm.nih.gov/pubmed/20364147 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1447 |
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