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Selection of genomic sequences that bind tightly to Ff gene 5 protein: primer-free genomic SELEX

Single-stranded DNA or RNA libraries used in SELEX experiments usually include primer-annealing sequences for PCR amplification. In genomic SELEX, these fixed sequences may form base pairs with the central genomic fragments and interfere with the binding of target molecules to the genomic sequences....

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Detalhes bibliográficos
Main Authors: Wen, Jin-Der, Gray, Donald M.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC545477/
https://ncbi.nlm.nih.gov/pubmed/15601993
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh179
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