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Low-fidelity Pyrococcus furiosus DNA polymerase mutants useful in error-prone PCR

Random mutagenesis constitutes an important approach for identifying critical regions of proteins, studying structure-function relations and developing novel proteins with desired properties. Perhaps, the most popular method is the error-prone PCR, in which mistakes are introduced into a gene, and h...

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Detalhes bibliográficos
Main Authors: Biles, Benjamin D., Connolly, Bernard A.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC545472/
https://ncbi.nlm.nih.gov/pubmed/15601989
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh174
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