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Low-fidelity Pyrococcus furiosus DNA polymerase mutants useful in error-prone PCR
Random mutagenesis constitutes an important approach for identifying critical regions of proteins, studying structure-function relations and developing novel proteins with desired properties. Perhaps, the most popular method is the error-prone PCR, in which mistakes are introduced into a gene, and h...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2004
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC545472/ https://ncbi.nlm.nih.gov/pubmed/15601989 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh174 |
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