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Improving dideoxynucleotide-triphosphate utilisation by the hyper-thermophilic DNA polymerase from the archaeon Pyrococcus furiosus

Polymerases from the Pol-I family which are able to efficiently use ddNTPs have demonstrated a much improved performance when used to sequence DNA. A number of mutations have been made to the gene coding for the Pol-II family DNA polymerase from the archaeon Pyrococcus furiosus with the aim of impro...

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Detalles Bibliográficos
Main Authors: Evans, Steven J., Fogg, Mark J., Mamone, Anthony, Davis, Maria, Pearl, Laurence H., Connolly, Bernard A.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2000
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC102620/
https://ncbi.nlm.nih.gov/pubmed/10666444
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