Purification and Characterization of a Membrane-Bound Hydrogenase from the Hyperthermophilic Archaeon Pyrococcus furiosus

Highly washed membrane preparations from cells of the hyperthermophilic archaeon Pyrococcus furiosus contain high hydrogenase activity (9.4 μmol of H(2) evolved/mg at 80°C) using reduced methyl viologen as the electron donor. The enzyme was solubilized with n-dodecyl-β-d-maltoside and purified by mu...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Sapra, Rajat, Verhagen, Marc F. J. M., Adams, Michael W. W.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2000
Assuntos:	Enzymes and Proteins
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC101913/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10852873/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.12.3423-3428.2000
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