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Purification and Characterization of a Membrane-Bound Hydrogenase from the Hyperthermophilic Archaeon Pyrococcus furiosus

Highly washed membrane preparations from cells of the hyperthermophilic archaeon Pyrococcus furiosus contain high hydrogenase activity (9.4 μmol of H(2) evolved/mg at 80°C) using reduced methyl viologen as the electron donor. The enzyme was solubilized with n-dodecyl-β-d-maltoside and purified by mu...

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Bibliografske podrobnosti
Main Authors: Sapra, Rajat, Verhagen, Marc F. J. M., Adams, Michael W. W.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2000
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC101913/
https://ncbi.nlm.nih.gov/pubmed/10852873
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