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Purification and Characterization of a Membrane-Bound Hydrogenase from the Hyperthermophilic Archaeon Pyrococcus furiosus
Highly washed membrane preparations from cells of the hyperthermophilic archaeon Pyrococcus furiosus contain high hydrogenase activity (9.4 μmol of H(2) evolved/mg at 80°C) using reduced methyl viologen as the electron donor. The enzyme was solubilized with n-dodecyl-β-d-maltoside and purified by mu...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC101913/ https://ncbi.nlm.nih.gov/pubmed/10852873 |
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