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Plasmid-based lacZα assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase
The preparation of a gapped pUC18 derivative, containing the lacZα reporter gene in the single-stranded region, is described. Gapping is achieved by flanking the lacZα gene with sites for two related nicking endonucleases, enabling the excision of either the coding or non-coding strand. However, the...
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| Autori principali: | , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2009
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2731893/ https://ncbi.nlm.nih.gov/pubmed/19515939 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp494 |
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