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Plasmid-based lacZα assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase

The preparation of a gapped pUC18 derivative, containing the lacZα reporter gene in the single-stranded region, is described. Gapping is achieved by flanking the lacZα gene with sites for two related nicking endonucleases, enabling the excision of either the coding or non-coding strand. However, the...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Jozwiakowski, Stanislaw K., Connolly, Bernard A.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2009
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2731893/
https://ncbi.nlm.nih.gov/pubmed/19515939
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp494
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