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Extensive libraries of gene truncation variants generated by in vitro transposition

The detailed analysis of the impact of deletions on proteins or nucleic acids can reveal important functional regions and lead to variants with improved macromolecular properties. We present a method to generate large libraries of mutants with deletions of varying length that are randomly distribute...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Morelli, Aleardo, Cabezas, Yari, Mills, Lauren J., Seelig, Burckhard
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5449547/
https://ncbi.nlm.nih.gov/pubmed/28130425
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx030
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