Lanean...
Non-radioactive in vitro PINK1 Kinase Assays Using Ubiquitin or Parkin as Substrate
This protocol describes the in vitro phosphorylation of ubiquitin and Parkin by the kinase PINK1 using recombinant proteins. Both substrates, ubiquitin and Parkin, are phosphorylated at the conserved serine 65 residue (pS65-ubiquitin and pS65-Parkin). The protocol also includes the use of monomeric...
Gorde:
| Argitaratua izan da: | Bio Protoc |
|---|---|
| Egile Nagusiak: | , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
2016
|
| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5448420/ https://ncbi.nlm.nih.gov/pubmed/28573163 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.1946 |
| Etiketak: |
Etiketa erantsi
Etiketarik gabe, Izan zaitez lehena erregistro honi etiketa jartzen!
|