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Non-radioactive in vitro PINK1 Kinase Assays Using Ubiquitin or Parkin as Substrate
This protocol describes the in vitro phosphorylation of ubiquitin and Parkin by the kinase PINK1 using recombinant proteins. Both substrates, ubiquitin and Parkin, are phosphorylated at the conserved serine 65 residue (pS65-ubiquitin and pS65-Parkin). The protocol also includes the use of monomeric...
Tallennettuna:
| Julkaisussa: | Bio Protoc |
|---|---|
| Päätekijät: | , , |
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
2016
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5448420/ https://ncbi.nlm.nih.gov/pubmed/28573163 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.1946 |
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