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Metabolic Labeling and Recovery of Nascent RNA to Accurately Quantify mRNA Stability

Changes in the rate of mRNA decay are closely coordinated with transcriptional changes and together these events have profound effects on gene expression during development and disease. Traditional approaches to assess mRNA decay have relied on inhibition of transcription, which can alter mRNA decay...

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Detalhes bibliográficos
Publicado no:Methods
Main Authors: Russo, Joseph, Heck, Adam M., Wilusz, Jeffrey, Wilusz, Carol J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5447484/
https://ncbi.nlm.nih.gov/pubmed/28219744
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2017.02.003
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