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Deep interactome profiling of membrane proteins by Co-interacting Protein Identification Technology (CoPIT)

Affinity purification coupled to mass spectrometry (AP-MS) is the method of choice to analyze protein-protein interactions, but common protocols frequently recover only the most stable interactions and tend to result in low bait yield for membrane proteins. Here, we present a novel, deep interactome...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Pankow, Sandra, Bamberger, Casimir, Calzolari, Diego, Bamberger, Andreas, Yates, John R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5444904/
https://ncbi.nlm.nih.gov/pubmed/27854364
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.140
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