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Highly sensitive and multiplexed quantification of mRNA splice variants by the direct ligation of DNA probes at the exon junction and universal PCR amplification

Alternative messenger RNA (mRNA) splicing is a basic mechanism of gene regulation. In general, reverse transcription and polymerase based primer extension limit the sensitivity and selectivity of the current detection of mRNA splice variants, respectively. Here, we show that, using the ligation of t...

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書目詳細資料
發表在:Chem Sci
Main Authors: Wang, Honghong, Wang, Hui, Duan, Xinrui, Sun, Yuanyuan, Wang, Xiangdong, Li, Zhengping
格式: Artigo
語言:Inglês
出版: Royal Society of Chemistry 2017
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5437374/
https://ncbi.nlm.nih.gov/pubmed/28580102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7sc00094d
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