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Highly sensitive and multiplexed quantification of mRNA splice variants by the direct ligation of DNA probes at the exon junction and universal PCR amplification

Alternative messenger RNA (mRNA) splicing is a basic mechanism of gene regulation. In general, reverse transcription and polymerase based primer extension limit the sensitivity and selectivity of the current detection of mRNA splice variants, respectively. Here, we show that, using the ligation of t...

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Dettagli Bibliografici
Pubblicato in:Chem Sci
Autori principali: Wang, Honghong, Wang, Hui, Duan, Xinrui, Sun, Yuanyuan, Wang, Xiangdong, Li, Zhengping
Natura: Artigo
Lingua:Inglês
Pubblicazione: Royal Society of Chemistry 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5437374/
https://ncbi.nlm.nih.gov/pubmed/28580102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7sc00094d
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