Isolation and Partial Characterization of Vacuoles from Tobacco Protoplasts
Protoplasts from suspension-cultured cells of Nicotiana glutinosa L. were lysed in 0.3 molar sorbitol in 2 millimolar ethylenediaminetetraacetate-tris(hydroxymethyl) aminomethane (pH 7.5) to release intact vacuoles. The vacuoles were purified by centrifugation in a Ficoll step gradient. About 11% of...
Salvato in:
| Pubblicato in: | Plant Physiol |
|---|---|
| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1979
|
| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC543202/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16661103/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.64.6.1114 |
| Tags: |
Nessun Tag, puoi essere il primo ad aggiungerne!!
|