Isolation and Partial Characterization of Vacuoles from Tobacco Protoplasts
Protoplasts from suspension-cultured cells of Nicotiana glutinosa L. were lysed in 0.3 molar sorbitol in 2 millimolar ethylenediaminetetraacetate-tris(hydroxymethyl) aminomethane (pH 7.5) to release intact vacuoles. The vacuoles were purified by centrifugation in a Ficoll step gradient. About 11% of...
Guardat en:
| Publicat a: | Plant Physiol |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1979
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC543202/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16661103/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.64.6.1114 |
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