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An improved method with high sensitivity and low background in detecting low β-galactosidase expression in mouse embryos
LacZ is widely used as a reporter in studies of gene expression patterns. β-galactosidase, the product of LacZ gene, is usually detected by X-gal/FeCN staining. In X-gal/FeCN staining, β-galactosidase catalyzes X-gal to produce blue precipitates, which indicate the expression patterns of the gene of...
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5419561/ https://ncbi.nlm.nih.gov/pubmed/28475610 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0176915 |
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