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A fast and sensitive alternative for β-galactosidase detection in mouse embryos

The bacterial lacZ gene is widely used as a reporter in a myriad of mouse transgenic experiments. β-Galactosidase, encoded by lacZ, is usually detected using X-gal in combination with ferric and ferrous ions. This assay produces a blue indole precipitate that is easy to detect visually. Here, we sho...

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Detalhes bibliográficos
Main Authors: Sundararajan, Sakthi, Wakamiya, Maki, Behringer, Richard R., Rivera-Pérez, Jaime A.
Formato: Artigo
Idioma:Inglês
Publicado em: Company of Biologists 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3509737/
https://ncbi.nlm.nih.gov/pubmed/23132248
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.078790
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