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Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

The Escherichia coli LacZ gene, encoding β-galactosidase, is largely used as a reporter for gene expression and as a tracer in cell lineage studies. The classical histochemical reaction is based on the hydrolysis of the substrate X-gal in combination with ferric and ferrous ions, which produces an i...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: Blanco, María José, Learte, Ana I.R., Marchena, Miguel A., Muñoz-Sáez, Emma, Cid, María Antonia, Rodríguez-Martín, Iván, Sánchez-Camacho, Cristina
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101999/
https://ncbi.nlm.nih.gov/pubmed/30010638
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57785
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