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Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
The Escherichia coli LacZ gene, encoding β-galactosidase, is largely used as a reporter for gene expression and as a tracer in cell lineage studies. The classical histochemical reaction is based on the hydrolysis of the substrate X-gal in combination with ferric and ferrous ions, which produces an i...
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| Опубликовано в: : | J Vis Exp |
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| Главные авторы: | , , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
MyJove Corporation
2018
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6101999/ https://ncbi.nlm.nih.gov/pubmed/30010638 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57785 |
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