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The Stem-Loop Luciferase Assay for Polyadenylation (SLAP) Method for Determining CstF-64-Dependent Polyadenylation Activity

Polyadenylation is an essential cellular process in eukaryotic cells (Edmonds M and Abrams R, J Biol Chem 235, 1142–1149, 1960; Zhao J et al., Microbiol Mol Biol Rev 63, 405–445, 1999; Edmonds M, Progr Nucleic Acid Res Mol Biol 71, 285–389, 2002). For this reason, it has been difficult to examine th...

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書目詳細資料
發表在:Methods Mol Biol
Main Authors: Hockert, J. Andrew, MacDonald, Clinton C.
格式: Artigo
語言:Inglês
出版: 2014
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5417545/
https://ncbi.nlm.nih.gov/pubmed/24590783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-971-0_9
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