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The Hinge Domain of the Cleavage Stimulation Factor Protein CstF-64 Is Essential for CstF-77 Interaction, Nuclear Localization, and Polyadenylation
Because polyadenylation is essential for cell growth, in vivo examination of polyadenylation protein function has been difficult. Here we describe a new in vivo assay that allows structure-function assays on CstF-64, a protein that binds to pre-mRNAs downstream of the cleavage site for accurate and...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Biochemistry and Molecular Biology
2010
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2804217/ https://ncbi.nlm.nih.gov/pubmed/19887456 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.061705 |
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