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A fully automated procedure for the parallel, multidimensional purification and nucleotide loading of the human GTPases KRas, Rac1 and RalB

Small GTPases regulate many key cellular processes and their role in human disease validates many proteins in this class as desirable targets for therapeutic intervention. Reliable recombinant production of GTPases, often in the active GTP loaded state, is a prerequisite for the prosecution of drug...

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Detalhes bibliográficos
Publicado no:Protein Expr Purif
Main Authors: Gray, Christopher H., Konczal, Jennifer, Mezna, Mokdad, Ismail, Shehab, Bower, Justin, Drysdale, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: Academic Press 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5415301/
https://ncbi.nlm.nih.gov/pubmed/28137655
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2017.01.010
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