Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate

The guanine nucleotide exchange factor (GEF) Son of Sevenless (SOS) plays a critical role in signal transduction by activating Ras. Here we introduce a single-molecule assay in which individual SOS molecules are captured from raw cell lysate using Ras-functionalized supported membrane microarrays. T...

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Bibliografske podrobnosti
izdano v:Nat Commun
Main Authors: Lee, Young Kwang, Low-Nam, Shalini T., Chung, Jean K., Hansen, Scott D., Lam, Hiu Yue Monatrice, Alvarez, Steven, Groves, Jay T.
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 2017
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5414354/
https://ncbi.nlm.nih.gov/pubmed/28452363
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms15061
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