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Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

B cells reactive with a specific antigen usually occur at a frequency of <0.05% of lymphocytes. For decades researchers have sought methods to isolate and enrich these rare cells for studies of their phenotype and biology. Approaches are inevitably based on the principle that B cells recognize na...

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Podrobná bibliografie
Vydáno v:J Vis Exp
Hlavní autoři: Smith, Mia J., Packard, Thomas A., O'Neill, Shannon K., Hinman, Rochelle M., Rihanek, Marynette, Gottlieb, Peter A., Cambier, John C.
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5409333/
https://ncbi.nlm.nih.gov/pubmed/28287549
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55382
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