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Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
B cells reactive with a specific antigen usually occur at a frequency of <0.05% of lymphocytes. For decades researchers have sought methods to isolate and enrich these rare cells for studies of their phenotype and biology. Approaches are inevitably based on the principle that B cells recognize na...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5409333/ https://ncbi.nlm.nih.gov/pubmed/28287549 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55382 |
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