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Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
This manuscript describes a protocol for detecting transcription termination defect in vivo. The strand-specific TRO protocol using BrUTP described here is a powerful experimental approach for analyzing the transcription termination defect under physiological conditions. Like the traditional TRO ass...
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| Publié dans: | J Vis Exp |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
MyJove Corporation
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5408991/ https://ncbi.nlm.nih.gov/pubmed/28362387 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55446 |
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