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Effective Isolation of Functional Islets from Neonatal Mouse Pancreas

Perfusion-based islet-isolation protocols from large mammalian pancreata are well established. Such protocols are readily conducted in many laboratories due to the large size of the pancreatic duct that allows for ready collagenase injection and subsequent tissue perfusion. In contrast, islet isolat...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Huang, Chen, Gu, Guoqiang
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5408776/
https://ncbi.nlm.nih.gov/pubmed/28117808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55160
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