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RNA Isolation from Mouse Pancreas: A Ribonuclease-rich Tissue
Isolation of high-quality RNA from ribonuclease-rich tissue such as mouse pancreas presents a challenge. As a primary function of the pancreas is to aid in digestion, mouse pancreas may contain as much a 75 mg of ribonuclease. We report modifications of standard phenol/guanidine thiocyanate lysis re...
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Publicado no: | J Vis Exp |
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Main Authors: | , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
MyJove Corporation
2014
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4297470/ https://ncbi.nlm.nih.gov/pubmed/25145327 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51779 |
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