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Effective Isolation of Functional Islets from Neonatal Mouse Pancreas

Perfusion-based islet-isolation protocols from large mammalian pancreata are well established. Such protocols are readily conducted in many laboratories due to the large size of the pancreatic duct that allows for ready collagenase injection and subsequent tissue perfusion. In contrast, islet isolat...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Huang, Chen, Gu, Guoqiang
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5408776/
https://ncbi.nlm.nih.gov/pubmed/28117808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55160
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