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Soluble expression of recomb inant cMyc, Klf4, Oct4, and Sox2 proteins in bacteria and transduction into living cells

AIM: To develop a new method to produce recombinant reprogramming proteins, cMyc, Klf4, Oct4, and Sox2, in soluble format with low cost for the generation of induced pluripotent stem cells (iPSCs). METHODS: A short polypeptide sequence derived from the HIV trans-activator of transcription protein (T...

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Detalhes bibliográficos
Publicado no:Int J Ophthalmol
Main Authors: Liu, Guo-Dan, Zhou, Shi-Feng, Ding, Xu-Chen, Fang, Chun-Lai, Mi, Shu-Yong, Gao, Xiang-Chun, Han, Qing
Formato: Artigo
Idioma:Inglês
Publicado em: International Journal of Ophthalmology Press 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5406633/
https://ncbi.nlm.nih.gov/pubmed/28503428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.18240/ijo.2017.04.10
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