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Effect of genetic modification of tyrosine-185 on the proton pump and the blue-to-purple transition in bacteriorhodopsin.

The retinylidene chromophore mutant (Y185F) of bacteriorhodopsin, in which Tyr-185 is substituted by phenylalanine, is examined and compared with wild-type bacteriorhodopsin expressed in Escherichia coli; both were reinstituted similarly in vesicles. The Y185F mutant shows (at least) two distinct sp...

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Bibliografiska uppgifter
Huvudupphovsmän: Jang, D J, el-Sayed, M A, Stern, L J, Mogi, T, Khorana, H G
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1990
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC54055/
https://ncbi.nlm.nih.gov/pubmed/2349220
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