Effect of genetic modification of tyrosine-185 on the proton pump and the blue-to-purple transition in bacteriorhodopsin.

The retinylidene chromophore mutant (Y185F) of bacteriorhodopsin, in which Tyr-185 is substituted by phenylalanine, is examined and compared with wild-type bacteriorhodopsin expressed in Escherichia coli; both were reinstituted similarly in vesicles. The Y185F mutant shows (at least) two distinct sp...

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Publié dans:	Proc Natl Acad Sci U S A
Auteurs principaux:	Jang, D J, el-Sayed, M A, Stern, L J, Mogi, T, Khorana, H G
Format:	Artigo
Langue:	Inglês
Publié:	National Academy of Sciences 1990
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54055/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2349220/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.11.4103
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