Effect of genetic modification of tyrosine-185 on the proton pump and the blue-to-purple transition in bacteriorhodopsin.
The retinylidene chromophore mutant (Y185F) of bacteriorhodopsin, in which Tyr-185 is substituted by phenylalanine, is examined and compared with wild-type bacteriorhodopsin expressed in Escherichia coli; both were reinstituted similarly in vesicles. The Y185F mutant shows (at least) two distinct sp...
Sparad:
| I publikationen: | Proc Natl Acad Sci U S A |
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| Huvudupphov: | , , , , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Utgiven: |
National Academy of Sciences
1990
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54055/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2349220/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.11.4103 |
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