Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli

BACKGROUND: The exploitation of the CRISPR/Cas9 machinery coupled to lambda (λ) recombinase-mediated homologous recombination (recombineering) is becoming the method of choice for genome editing in E. coli. First proposed by Jiang and co-workers, the strategy has been subsequently fine-tuned by seve...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Microb Cell Fact
Main Authors: Zerbini, Francesca, Zanella, Ilaria, Fraccascia, Davide, König, Enrico, Irene, Carmela, Frattini, Luca F., Tomasi, Michele, Fantappiè, Laura, Ganfini, Luisa, Caproni, Elena, Parri, Matteo, Grandi, Alberto, Grandi, Guido
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2017
Teme:
Research
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5404680/
https://ncbi.nlm.nih.gov/pubmed/28438207
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-017-0681-1
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