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Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli
BACKGROUND: The exploitation of the CRISPR/Cas9 machinery coupled to lambda (λ) recombinase-mediated homologous recombination (recombineering) is becoming the method of choice for genome editing in E. coli. First proposed by Jiang and co-workers, the strategy has been subsequently fine-tuned by seve...
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| Pubblicato in: | Microb Cell Fact |
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| Autori principali: | , , , , , , , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
BioMed Central
2017
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5404680/ https://ncbi.nlm.nih.gov/pubmed/28438207 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-017-0681-1 |
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