Introduction of large linear minichromosomes into Schizosaccharomyces pombe by an improved transformation procedure.

The efficiency of transformation of Schizosaccharomyces pombe has been increased 10- to 50-fold over previously reported methods. By using 1 microgram of plasmid, 7.0 x 10(5) transformants are regularly obtained. This increased transformation efficiency is mainly due to the inclusion of the cationic...

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Detaylı Bibliyografya
Yazar: Allshire, R C
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1990
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC54043/
https://ncbi.nlm.nih.gov/pubmed/2349217
Etiketler: Etiketle
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