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Introduction of large linear minichromosomes into Schizosaccharomyces pombe by an improved transformation procedure.

The efficiency of transformation of Schizosaccharomyces pombe has been increased 10- to 50-fold over previously reported methods. By using 1 microgram of plasmid, 7.0 x 10(5) transformants are regularly obtained. This increased transformation efficiency is mainly due to the inclusion of the cationic...

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Autor principal: Allshire, R C
Format: Artigo
Idioma:Inglês
Publicat: 1990
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC54043/
https://ncbi.nlm.nih.gov/pubmed/2349217
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