Introduction of large linear minichromosomes into Schizosaccharomyces pombe by an improved transformation procedure.
The efficiency of transformation of Schizosaccharomyces pombe has been increased 10- to 50-fold over previously reported methods. By using 1 microgram of plasmid, 7.0 x 10(5) transformants are regularly obtained. This increased transformation efficiency is mainly due to the inclusion of the cationic...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54043/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2349217/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.11.4043 |
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