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Interaction and mutual stabilization of the two subunits of vaccinia virus mRNA capping enzyme coexpressed in Escherichia coli.

The genes D1 and D2, predicted to encode the 95- and 31-kDa subunits of the vaccinia virus mRNA capping enzyme, were coexpressed from the same plasmid in Escherichia coli. Induction with low concentrations of isopropyl beta-D-thiogalactoside was necessary to obtain soluble enzyme. The active heterod...

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Dades bibliogràfiques
Publicat a:Proc Natl Acad Sci U S A
Autors principals: Guo, P X, Moss, B
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 1990
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54039/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2161527/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.11.4023
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