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Interaction and mutual stabilization of the two subunits of vaccinia virus mRNA capping enzyme coexpressed in Escherichia coli.

The genes D1 and D2, predicted to encode the 95- and 31-kDa subunits of the vaccinia virus mRNA capping enzyme, were coexpressed from the same plasmid in Escherichia coli. Induction with low concentrations of isopropyl beta-D-thiogalactoside was necessary to obtain soluble enzyme. The active heterod...

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Bibliografske podrobnosti
Main Authors: Guo, P X, Moss, B
Format: Artigo
Jezik:Inglês
Izdano: 1990
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC54039/
https://ncbi.nlm.nih.gov/pubmed/2161527
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